Journal: Journal of Biological Engineering

Article Title: Bioengineered injectable HAMA/GelMA hydrogel encapsulating exosomes loaded lycopene mitigates deoxynivalenol-induced testicular injury via apoptotic pathway modulation

doi: 10.1186/s13036-025-00555-3

Figure Lengend Snippet: Apoptotic signaling pathways of Protein and mRNA expression: A & B ) Caspase-3 activity; C & D ) Bax; E & F ) Bcl-2; G & H ) Cytc. Data are presented as mean ± SD, ( n = 10). ** P < 0.01 vs. control, # P < 0.05 vs. Dex, ## P < 0.01 vs. Dex

Article Snippet: The reaction was conducted at 25 °C (10 min), 42 °C (50 min), and 70 °C (15 min), then immediately cooled on ice. qRT-PCR amplification was conducted using gene-specific primers (listed in Table ) for Caspase-3, Bax, Bcl-2, and Cytochrome c (Cytc), with SYBR Green Master Mix (Thermo Scientific, USA) on a real-time PCR system.

Techniques: Protein-Protein interactions, Expressing, Activity Assay, Control

Journal: Journal of Biological Engineering

Article Title: Bioengineered injectable HAMA/GelMA hydrogel encapsulating exosomes loaded lycopene mitigates deoxynivalenol-induced testicular injury via apoptotic pathway modulation

doi: 10.1186/s13036-025-00555-3

Figure Lengend Snippet: Protein and mRNA gene expression by qRT-PCR A & B ) Caspase-3 activity; C & D ) Bax; E & F ) Bcl-2; G & H ) Cytc. Data are presented as mean ± SD, ( n = 10). ** P < 0.01 vs. control, # P < 0.05 vs. Dex

Article Snippet: The reaction was conducted at 25 °C (10 min), 42 °C (50 min), and 70 °C (15 min), then immediately cooled on ice. qRT-PCR amplification was conducted using gene-specific primers (listed in Table ) for Caspase-3, Bax, Bcl-2, and Cytochrome c (Cytc), with SYBR Green Master Mix (Thermo Scientific, USA) on a real-time PCR system.

Techniques: Gene Expression, Quantitative RT-PCR, Activity Assay, Control